Table 2.
Line | LMF IC50, nmol/La |
Recombinant | Parental line |
PfCRT haplotype | CQb | ARTM IC50,b nmol/L |
MQ IC50,b nmol/L |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
72 | 74 | 75 | 76 | 220 | 271 | 326 | 356 | 371 | IC50, nmol/L | Phenotype | ||||||
GC03 | 85.5 ± 7.2 | No | … | C | M | N | K | A | Q | N | I | R | 29.2 ± 2.0 | Susc. | 39.4 ± 6.9 | 52.6 ± 5.4 |
Dd2 | 55.2 ± 9.6 | No | … | C | I | E | T | S | E | S | T | I | 195.1 ± 25.3 | Res. | 46.3 ± 7.6 | 46.8 ± 2.0 |
7G8 | 27.0 ± 4.1 | No | … | S | M | N | T | S | Q | D | L | R | 140.4 ± 15.0 | Res. | 21.6 ± 6.3 | 9.6 ± 1.3 |
C2GC03 | 74.0 ± 7.7 | Yes | GC03 | C | M | N | K | A | Q | N | I | R | 22.9 ± 2.8 | Susc. | 39.1 ± 7.0 | 54.7 ± 1.5 |
C4Dd2 | 48.7 ± 4.3c | Yes | GC03 | C | I | E | T | S | E | S | T | I | 147.4 ± 13.3d | Res. | 12.6 ± 1.8d | 33.1 ± 3.1d |
C67G8 | 46.3 ± 4.6d | Yes | GC03 | S | M | N | T | S | Q | D | L | R | 126.9 ± 17.2d | Res. | 17.2 ± 3.0d | 27.3 ± 3.4d |
T76K-1Dd2 | 73.2 ± 10.5d | Yes | Dd2 | C | I | E | K | S | E | S | T | I | 22.1 ± 2.1d | Susc. | 40.4 ± 11.7 | 27.2 ± 8.0c |
C-1Dd2 | 33.7 ± 5.0 | Yes | Dd2 | C | I | E | T | S | E | S | T | I | 160.7 ± 28.9 | Res. | 32.9 ± 6.6 | 36.9 ± 4.2 |
T76K-17G8 | 68.3 ± 11.7d | Yes | 7G8 | C | I | E | K | S | Q | D | L | R | 23.9 ± 4.6e | Susc. | 13.8 ± 3.2 | 3.6 ± 0.7 |
C-17G8 | 33.6 ± 5.5 | Yes | 7G8 | S | M | N | T | S | Q | D | L | R | 108.8 ± 15.9 | Res. | 7.4 ± 2.1 | 3.1 ± 0.9 |
NOTE. ARTM, artemisinin; CQ, chloroquine; LMF, lumefantrine; MQ, mefloquine; Res., resistant; Susc., susceptible.
LMF IC50 values are expressed as the mean value (±SE) for pfcrt–modified and control lines. Data were derived from 5–12 independent assays performed in duplicate. (Results from Mann-Whitney U tests compared mutants and recombinant controls).
CQ, ARTM, and MQ IC50 values (from at least 3 independent assays performed in duplicate) are expressed as the mean value (±SE) and are reproduced from [19] for the top 6 lines and from [20] for the bottom 4 lines.
P < .05.
P < .01.
P < .001.