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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: J Immunol. 2009 Jan 15;182(2):1146–1154. doi: 10.4049/jimmunol.182.2.1146

Figure 2. CARD9 is recruited to forming phagosomes during Dectin-1-mediated phagocytosis, but is not required for phagocytosis.

Figure 2

(A) Full length CARD9, the CARD domain of CARD9, and the CARD9 coiled-coil domain were tagged with streptavidin-binding peptide (SBPc). (B) RAW264.7 macrophages stably expressing SBPc-tagged full length CARD9, CARD domain, or coiled-coil domain were fed 100 μg/ml zymosan particles for 10 min, prior to fixing and staining with an antibody against the SBPc tag (CARD9 constructs) and TRITC-phalloidin (β-actin). Arrows identify actin-positive early phagosomes; arrowheads identify actin-negative later phagosomes. (C) Bone marrow-derived macrophages (bmM) and dendritic cells (bmDC) from wild type and CARD9-deficient mice were fed 100 μg/ml TRITC-labeled zymosan for the times indicated and phagocytosis was assessed by flow cytometry.