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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Immunol. 2008 Dec 1;181(11):7825–7834. doi: 10.4049/jimmunol.181.11.7825

Table III.

Frequency of VH4-59 products from CD19 CD19− cells

Gene CD19− CD19+
IGHV4-b 0 0
IGHV4-04 1/96 (1%) 7/55 (13%)
IGHV4-34 24/96 (25%) 12/55 (22%)
IGHV4-39 0 12/55 (22%)
IGHV4-59 71/96 (74%) 12/55 (33%)
VH4 hybrids 0 7/69 (10%)

CD19− and CD19+ cells were isolated from PBMCs from a single donor. Nested PCR reactions were performed on both populations. The nested primers used on the CD19+ cells were designed to amplify all rearranged VH4 genes and the nested primers used on the CD19− cells preferentially amplified unrearranged VH4-59 gene segments in the heavy chain locus.