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. 2000 Nov 7;97(24):13108–13113. doi: 10.1073/pnas.240454997

Figure 4.

Figure 4

In situ fluorescence of the mutant strain CBF5-AUU and the parental wild-type strain. DAPI stains of the nucleoplasm are blue in all views. The fluorescein-labeled probe to the introns of pre-tRNAs are green, and the Cy3-labeled probe to U14 nucleolar RNA is red. In the wild-type strain, most of the pre-tRNA signal is colocalized to the nucleolus, along with the U14 snoRNA, although there are some secondary loci of pre-tRNA staining. In the CBF5-AUU strain, the U14 nucleolar signal is slightly fragmented, but the pre-tRNA signal is dispersed to the nucleoplasm.