Figure 2. Recursive partitioning mixture models (RPMM) of methylation profiles in normal tissuses.

Methylation average β is yellow for unmethylated and blue for methylated. Methylation profile classes are stacked in rows separated by red lines, and class height corresponds to the number of samples in each class. Class methylation at each CpG locus is a mean of methylation for all samples within a class. (A) Methylation profile classes significantly differentiate all normal tissue types (n = 217, P<0.0001). (B) Methylation profile classes from RPMM of adult bloods, class membership is significantly associated with age (P<0.005, n = 30). (C) Methylation profile classes from RPMM of lung tissue samples (n = 53). (D) Methylation profile classes from RPMM of pleural samples (n = 18).