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. 2009 Aug 10;4(8):e6566. doi: 10.1371/journal.pone.0006566

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Yau et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Comparison of sizes of DLC2+/+ (n = 6) and DLC2−/− (n = 6) cells by flow cytometry. Forward light scatter (FSC) of G1-phase-cells as determined by flow cytometry was used to measure the relative cell sizes. Mean values±standard errors and p-values are shown. P-values were calculated by unpaired Student's t test. (b) Representative samples of flow cytometry results. (c) Western blot analysis of insulin-treated DLC2+/+ and DLC2−/− cells. (d) RhoA activity of DLC2+/+ and DLC2−/− cells as detected by Rhotekin binding assay. (e) Actin cytoskeleton and focal adhesions in DLC2+/+ and DLC2−/− MEF cells were detected by immunofluorescence staining of actin stress fibers (red) and Paxillin (green), respectively.