Figure 2.

Diplamine B (4) and lissoclinidine B (5) increased p53 and Hdm2 in cells in a dose-dependent manner. RPE cells were treated with 50 µM ALLN, or 1–10 µM 4 or 5 for 8 h. Total levels of p53, Hdm2 and β-actin, which serves as an equal loading control, were assessed by immunoblotting using antibodies directed against each of these proteins.