Figure 7.

A. Lissoclinidine B (5) selectively kills cells expressing wild-type p53. p53-deficient (A9) or wild-type p53 (C8) MEFs were treated with 5–20 µM 5 for 20 h. Cell death was measured by trypan blue exclusion. Data represents average and standard deviation of three independent experiments. B. Lissoclinidine B (5) induces apoptosis in C8 cells. A9 and C8 cells were incubated with 5–20 µM 5 for 14 h. Cellular PARP and β-actin were evaluated by immunoblotting using anti-PARP and anti-β-actin specific antibodies.