Table 1.

Steady-state kinetic parameters for ClpXP degradation of peptide substrates.

name	Vmax min−1 ClpP−1	KM µM	Vmax with SspB min−1 ClpP−1	cost ATP/peptide with SspB	Sequence	length
[G7]	13.7 ± 1.3	5.4 ± 1.9	13.5 ± 0.7	30	ABZ-FAPHMALVPYNO2G7KKAANDENYALAA	30
[G10]	14.3 ± 0.8	6.1 ± 0.2	11.5 ± 0.4	30	ABZ-FAPHMALVPYNO2G10KKAANDENYALAA	33
[G5KKG5]	14.5 ± 1.9	4.5 ± 2.2	12.1 ± 0.1	36	ABZ-FAPHMALVPYNO2G5KKG5AANDENYALAA	33
[β]10	9.8 ± 0.9	4.1 ± 0.3	8.8 ± 1.1	43	ABZ-FAPHMALVPYNO2β10KKAANDENYALAA ABZ-	33
[γ]10	6.4 ± 0.3	3.7 ± 0.6	6.5 ± 0.4	57	FAPHMALVPYNO2γ10KKAANDENYALAA ABZ-	33
[ε]10	6.1 ± 0.6	4.7 ± 0.4	5.9 ± 0.3	61	FAPHMALVPYNO2ε10KKAANDENYALAA	33
[O]5	7.0 ± 0.2	4.3 ± 0.4	6.8 ± 1.3	53	ABZ-FAPHMALVPYNO2O5KKAANDENYALAA ABZ-	33
[U]4	6.2 ± 0.6	9.0 ± 2.0	7.8 ± 0.5	47	FAPHMALVPYNO2U4KKAANDENYALAA	27
[P5]	11.4 ± 0.2	n.d.	12.9 ± 1.1	45	ABZ-FAPHMALVPYNO2P5KKAANDENYALAA ABZ-	28
[P10]	6.4 ± 0.2	2.8 ± 0.2	6.5 ± 0.2	65	FAPHMALVPYNO2P10KKAANDENYALAA ABZ-	33
[P15]	3.4 ± 0.8	3.6 ± 0.7	3.3 ± 0.3	125	FAPHMALVPYNO2P15KKAANDENYALAA	38
[VG]5	14.4 ± 1.7	3.4 ± 0.3	13.6 ± 0.4	30	ABZFAPHMALVPYNO2 [VG]5KKAANDENYALAA	33
[DVG]5	14.0 ± 0.4	6.7 ± 1.6	14.2 ± 0.2	28	ABZ-FAPHMALVPYNO2[dVG]5KKAANDENYALAA	33
[FG]5	12.1 ± 1.7	4.2 ± 1.0	14.1 ± 0.8	22	ABZ-FAPHMALVPY [FG]5KKAANDENYALAA	33
[Q10]	8.7 ± 0.5	9.1 ± 1.7	8.8 ± 0.8	48	ABZ-FAPHMALVPYNO2Q10KKAANDENYALAA ABZ-	33
[E10]	6.0 ± 0.1	2.0 ± 0.1	6.3 ± 0.2	62	FAPHMALVPYNO2E10KKAANDENYALAA ABZ-	33
[K10]	2.7 ± 0.1	• 0.2	2.8 ± 0.1	160	FAPHMALVPYNO2K10KKAANDENYALAA ABZ-	33
[R10]	8.0 ± 0.4	0.2 ± 0.1	8.4 ± 0.1	53	FAPHMALVPYNO2R10KKAANDENYALAA	33

β (β-alanine); γ (γ-aminobutyric acid), ε (ε-amino caproic acid); O (8-aminooctanoic acid), U (11-aminoundecanoic acid).

V_max and K_M values are means of 2–3 independent determinations (n) with errors calculated as $\sqrt{{\frac{1}{(n-1)}}_1^n{(\mathit{\text{value}}-\mathit{\text{mean}})}^2}$.