Table 1.

Summary of predicted STAT5 binding sites

Type	Predictions		Testedc	Verifiedc
	All sitesa	Sites found in all four speciesb
IGF-I
Paired 2 consensus	2	2	2	2
Paired 1 consensus	32	12	10	5
Paired 0 consensus	207	25	11	1
Single consensus	18	4	3	2
Single nonconsensus	115	16	0	0
Total	374	59	26	10
SOCS2
Paired 2 consensus	2	1	2	2
Paired 1 consensus	11	1	1	1
Paired 0 consensus	135	13	8	0
Single consensus	12	2	3	1
Single nonconsensus	80	3	2	0
Total	240	20	16	4
HNF6
Paired 2 consensus	1	0	1	0
Paired 1 consensus	17	3	4	1
Paired 0 consensus	163	16	16	2
Single consensus	9	1	1	0
Single nonconsensus	91	9	0	0
Total	281	29	22	3

For experimental validation, preference was given to the sites conserved in all four species (rat, mouse, human, and dog). Adjacent sites located in the proximity of amplicons for other sites were in many cases not considered if not found in all four species. 

^aSTAT5 sites predicted for the rat genes based on a set of nine TFBS matrices (see supplemental Table S1). 

^bPredicted rat STAT5 sites that are also present in mouse, human, and dog. 

^cTesting and verification is based on results for 57 amplicons (64 sites) presented in Fig. 1 and data in Fig. 4.