Figure 3.

Colocalization and evidence for interaction between AR and Runx2 in living cells. A, COS7 cells were transfected with the indicated plasmid/s (top), immunostained and analyzed by confocal microscopy to visualize the AR (red) and/or Runx2 (green). B, Runx2-GFP fusion protein was expressed in COS7 cells alone or together with WT AR or AR^A573D. The cells were treated with DHT or vehicle and subjected to FRAP analysis. Curves represent fluorescence intensity relative to the respective prephotobleaching levels. veh, Vehicle.