Figure 4.

CYP17A1 is regulated by PGC-1α through ERRα. A, HepG2 cells were transduced with lentiviruses expressing either a nonspecific control si (Csi), or siERRα, followed by adenoviral transduction of β-gal, PGC-1α, PGC-1α 2x9, or PGC-1α L2L3M. Gene expression of CYP17A1 was measured by qPCR and normalized to 36B4 expression. B, Schematic of the putative ERRE sites found in and around the CYP17A1 gene region. Regions not bound by ERRα are indicated by boxes with black hash marks. White boxes indicate the position of sites identified by ChIP scanning in C. Numbering is relative to the start site. C, ChIP of ERRα and amplification of putative ERREs by qPCR, where the ERRα promoter serves as a positive control.