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DHEA reduces the concentration of free amino acids in HepG2 cells. HepG2 cells were treated with 1 nm DHEA. Cell lysates were collected for analysis of free amino acid concentrations and normalized to total protein concentration. Error bars represent the se of three replicates, and the graph is representative of three independent experiments. Asterisks indicate significant difference (P < 0.05) by independent Student’s t test.
