Figure 5.
PTH causes Runx2 association with other proteins at the MMP-13 proximal promoter (−204/−34). Chromatin prepared from UMR cells, untreated and treated with rat PTH (peptide 1–34, 10−8 m) for 30 min or 120 min was immunoprecipitated with anti-Runx2, after which the immune complexes were collected then diluted with ChIP dilution buffer and resubjected to ChIP assays with anti-Runx2, anti-Ac-H3, anti-c-Jun, anti-p300-CT, or anti-CBP-NT antibodies. DNA was analyzed by PCR with primers to −204/−34 of the MMP-13 proximal promoter, and PCR products were separated on 2% agarose gels. Ac, Acetylated.