Table 1.
Antigen | Clone | Dilution | Antigen Retrieval* | Source |
---|---|---|---|---|
CD20 | L26 | 1:1,200 | MW, CB | DAKO, Carpinteria, CA |
CD3 | SP7 | 1:200 | S, CB | NeoMarkers/Lab Vision, Fremont, CA |
CD10 | 56C6 | 1:100 | S, CB | Novocastra, Newcastle upon Tyne, England |
bcl-2 | 124 | 1:400 | MW, CB | DAKO |
bcl-6 | PG-B6P | 1:100 | T + S, TRIS | DAKO |
Ki-67 | MIB-1 | 1:4,800 | PC, CB | DAKO |
LANA of human herpesvirus 8 (ORF-73) | LN-53 | 1:30,000 | PC, CB | Advanced Biotechnologies, Columbia, MD |
TdT | Polyclonal | 1:1,600 | PC, EDTA | DAKO |
LMP1 | CS1-4 | 1:500 | S, CB | DAKO |
CB, citrate buffer, pH 6; LANA, latent nuclear antigen; MW, microwave oven; ORF, open reading frame; PC, pressure cooker; S, steamer; T, trypsin; TdT, terminal deoxynucleotidyl transferase; TRIS, tris(hydroxymethyl)aminomethane.
Heat-induced epitope retrieval was used.