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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Acquir Immune Defic Syndr. 2009 Jun 1;51(2):125–129. doi: 10.1097/QAI.0b013e31819f9e31

Fig. 1. Activity of PRO 2000 in saline and in the presence of cervicovaginal lavage fluid (CVL).

Fig. 1

Saline or CVL from healthy donors was mixed with 1, 10, or 100 µg/ml of PRO 2000 for 5 min then incubated with U87-CD4-CCR5 cells for 1 h at 37°C, followed by 2 h infection with a HIV-1 pseudotyped subtype B or subtype C from recently infected patients. The cells were washed with DMEM and cultured in fresh medium for 48 h. Single-cycle HIV-1 infection was determined by lysing cells to measure luciferase production. The concentration of 100 µg/ml of PRO 2000 inhibited infection of U87-CD4-CCR5 cells mediated by envelopes derived from recently transmitted B and C viruses in saline and CVL. Data represent the mean +/− SD for virus tested. Each condition was tested in triplicate. RLU, relative light units.