Table 2.

ATP Concentration Values Obtained With 10 nM 1,25D Compared With Vehicle in Three Osteoblastic Cell Systems Studied

	Basal	1,25D	Fold increase
SAOS-2	2.75 ± 0.39	16.93 ± 1.83*	6.2 ± 0.3
Si RNA VDR SAOS-2	2.42 ± 0.390.23	2.81 ± 0.33	1.2 ± 0.1
ROS 17/2.8	6.09 ± 0.58	18.89 ± 1.39*	3.1 ± 0.2
Si RNA ROS 17/2.8	5.71 ± 0.39 0.36	7.08 ± 0.39 0.41	1.2 ± 0.1
Primary osteoblasts	3.06 ± 0.41	12.73 ± 0.52*	4.2 ± 0.3
VDR KO primary osteoblasts	3.43 ± 0.24	4.25 ± 0.43	1.2 ± 0.2

ATP concentration is expressed in nM and has been normalized to total protein concentration in the cell samples. Basal corresponds to vehicle control (ethanol 0.01%), and 12,5D concentration is 10 nM. Data are mean values ± SE of n = 18–48 experiments.

* p < 0.01.