Table 2.
Basal | 1,25D | Fold increase | |
SAOS-2 | 2.75 ± 0.39 | 16.93 ± 1.83* | 6.2 ± 0.3 |
Si RNA VDR SAOS-2 | 2.42 ± 0.390.23 | 2.81 ± 0.33 | 1.2 ± 0.1 |
ROS 17/2.8 | 6.09 ± 0.58 | 18.89 ± 1.39* | 3.1 ± 0.2 |
Si RNA ROS 17/2.8 | 5.71 ± 0.39 0.36 | 7.08 ± 0.39 0.41 | 1.2 ± 0.1 |
Primary osteoblasts | 3.06 ± 0.41 | 12.73 ± 0.52* | 4.2 ± 0.3 |
VDR KO primary osteoblasts | 3.43 ± 0.24 | 4.25 ± 0.43 | 1.2 ± 0.2 |
ATP concentration is expressed in nM and has been normalized to total protein concentration in the cell samples. Basal corresponds to vehicle control (ethanol 0.01%), and 12,5D concentration is 10 nM. Data are mean values ± SE of n = 18–48 experiments.
* p < 0.01.