Fig. 1.

(A) Analysis of cell-surface phenotypes of Mtb- and BCG-infected MoDC. Cells were infected for 24 h at a MOI of 1, and the cell-surface expression of CD86, CD83, HLA-DR, and CD38 was analyzed by flow cytometry. A total of 5000 cells was analyzed per sample. Unstimulated and infected cells stained with a control (Ctr) Ab are represented by the M1 bar. The results shown are from one of three experiments that yielded similar results. (B) Analysis of Mtb and BCG infection and intracellular survival. DC (5×10⁵) were lysed, and samples were plated to determine the number of bacterial CFU at various times after infection (0, 4, and 20 h). The results represent the means ± se of three independent experiments.