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. 2009 Apr 1;85(6):928–938. doi: 10.1189/jlb.1008621

Fig. 6.

Fig. 6.

Age-related increase in axin expression is prevented by chronic CR. (A) Quantitation of axin expression by Western blot of total thymic protein lysates displayed a marked increase in axin protein expression with age, and CR maintains the thymic axin to the levels observed in young animals. (B) The real-time PCR analysis of thymic axin mRNA in young and old mice. The age-matched CR mouse thymi revealed significantly (P<0.05) reduced axin expression in 12- and 18-month thymi (n=6/group). The data are expressed as mean ± sem. (C) The splenic CD4 cells were isolated to prepare the DNA. Compared with AL-fed 4-month-old mice, the 10-month-old animals displayed a significant reduction in the sjTRECs, suggesting a reduced thymic cell output. The 10-month-old mice on a CR diet displayed a significant increase in the sjTRECs. A total of eight mice/group was used for TREC real-time PCR analysis. The data are expressed as mean ± sem.