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. 2009 Aug 7;4(8):e6546. doi: 10.1371/journal.pone.0006546

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Gunnersen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rat retina vertical sections double stained for Sez-6 (red) and (in green): (A) GABA – non-GABAergic amacrine and putative displaced amacrine cells are marked (arrowheads) while double-labelled amacrine cells are marked with asterisks. (B) Parvalbumin (PV) – Sez-6 positive amacrine cells with their somata located in the second and third layer from the IPL (arrowheads) and double-stained cells with the elongated soma typical of bipolar cells (arrows) are observed. (C) Choline acetyltransferase (ChAT)-positive amacrines in the INL and displaced amacrines in the GCL were double-labelled with Sez-6. (D) Dopaminergic amacrine cells (labelled with tyrosine hydroxylase) were rarely found however they co-labelled for Sez-6. (E) Brain nitric oxide synthase (bNOS) amacrine cells were also positive for Sez6 staining. (F & G) Immunoreactivity for Protein kinase C alpha (PKCα - green) in rod bipolar cells overlaid on a bright field image (F) or shown with Sez-6 in red (G) indicating Sez6 staining at the apical pole of the cell soma (arrowheads). Scale bars: 25 µm.