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. Author manuscript; available in PMC: 2009 Jul 30.
Published in final edited form as: ACS Nano. 2008 Aug;2(8):1645–1652. doi: 10.1021/nn800370j

Figure 3.

Figure 3

(A) Illustration depicting the experimental setup used for photothermal therapy of SK-BR-3 cells. The cellular growth vessel had a diameter of 6.38 mm. The cells were irradiated with an 805 nm Ti:sapphire laser with a spot size of 2 mm. Thus, only 9.8% of the cells in the well were exposed to the laser. Note: cells not drawn to scale. (B) Plots of the percentage of cellular damage versus harvest time when SK-BR-3 cells were irradiated for 5 min at a laser power of 4.77 W/cm2: ●, results for cells targeted with immuno Au nanocages; ◯, control (no incubation with immuno Au nanocages). (C) The flow cytometry graph analyzed to determine the percentage of cellular death after SK-BR-3 cells were incubated with immuno Au nanocages, irradiated for 5 min at a power density of 4.77 W/cm2, then harvested at 3 h for analysis. (D) Flow cytometry graph obtained from the control experiment in which SK-BR-3 cells were not treated with immuno Au nanocages but treated to similar laser treatment. For both (C) and (D), the signal in quadrant III corresponds to population of dead cells.