Figure 3.

Translational repression activity is distributed evenly throughout the oocyte. Prl and Prl-Vg1 transcripts at ≈20 nM were injected into stage VI oocytes (50 nl per oocyte), and the oocytes were incubated in MBSH for 12 hr at 18°C to ensure complete diffusion of the RNA within the oocytes. They were then labeled for 60 min with MBSH containing 1 μCi/ml [³⁵S]methionine, fixed, and then manually dissected into animal (A) and vegetal (V) halves. Either prolactin or Vg1 was then immunoprecipitated from each half and analyzed by SDS/PAGE and autoradiography.