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Deposition of purple acid phosphatase (PAP) and β-glucan on plasma membrane sheets during time course. Plasma membrane sheets were prepared from protoplasts cultured in the wall-regeneration medium for 0, 5, 15, 30, and 60 min. Purple acid phosphatase was detected with anti-wall-bound purple acid phosphate antibodies and β-glucan was stained with Calcofluor White. Each image was joined from five 0.6 μm optical section images. Bar = 50 μm.
