Table I.

Amount of extracellular polysaccharides formed by protoplasts at 60 h after onset of wall regeneration

Total polysaccharides of cell walls isolated from wild-type and transgenic protoplasts, and total polysaccharides secreted by wild-type and transgenic protoplasts into the cell wall regeneration medium were determined by the phenol-sulfuric acid method (see “Materials and Methods”). Average and sd values were obtained from three different experiments. PAP, Purple acid phosphatase.

Fraction	Wild Type	PAP Overexpression
	μg per mL cells
Wall	32 ± 4	61 ± 5
Medium	23 ± 2	52 ± 4