Figure 1.

Uncompartmented bioassay. Dual bioassay used to test the effect of truffle mycelial exudates/volatiles on plants. The lower half of the petri dish is filled with malt extract and inoculated with either mycelium or an agar plug (mock control) and kept in the dark at 23°C ± 2°C. After 10 d (to allow biomass buildup) seeds of the test plants are added to the upper part of the petri dish (on Murashige and Skoog agar). Note that the mycelium and plant roots are not in direct contact (in this picture, C. incanus seedlings are 10-d-old). Scale: Petri dish is 10 × 10 cm². [See online article for color version of this figure.]