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. 2009 Aug;150(4):1866–1879. doi: 10.1104/pp.109.136689

Table III.

Oligonucleotide primers for cloning and expression analysis

Name +/−a Sequence (5′ to 3′) Purpose
ms248 + GATGATTTTGGNGAYTTTRCWCC HCT cloning
ms252 AATNCMRSCWGGTCCCAT
SMART + TACGGCTGCGAGAAGACGACAGAAGGG HCT2 RACE
5′ primer + TACGGCTGCGAGAAGACGACAGAA
ms492 CCCTTATCTTTTGTGCTACATAG
ms493 CCTAAAGGCTTTAATGTGATTTCTC
ms521 + GATGTTAACTTAAACCATAATACC HCT2 cloning
ms490 GTTTGATAAATGCAAAGAACTAAACTTT
ms407 + GCGCGCCATATGATCATAAACGTGAGAGAT HCT1 expression
ms408 CGCGCGCTCGAGTTATCAGATATCATACAAGAATTCC
ms522 + GCCATGGTTACCATTAAAAATTCTTAC HCT2 expression
ms523 GCTCGAGTCATATATCCTCATAGAAGTAC
ms381 + CGCGAATGGACAACGAGTATT HCT1 q-rtPCR
ms382 TACCAAGATTCGGGCACTTGA
ms595 + ACACCCTCGAAAGTACCACGTT HCT2 q-rtPCR
ms596 TCTTCACTTGTGTCTTTTCTTCCTATAATG
ms597 + GACATGGACCAAAATACTCGTGATA EIF4A q-rtPCRb
ms598 CAGTTGTTATGAGCACTCGTGAAGA
a

Orientation: +, sense; −, antisense.

b

Based on GenBank accession BB919542.