Figure 7.

BT2 expression is modulated by multiple stress signals. A, Three-week-old wild-type plants were grown on MS or treated with either ABA (lane 2) or Me-JA (lane 3) for 3 h and subjected to either wounding stress (lane 4) or cold stress (lane 5) for 3 h. (B) The plants from A were then challenged with oxidative stress caused by either H₂O₂ or methyl viologen (MV) for the indicated times. Total RNA was extracted from rosette leaves and subjected to RNA-blot analysis. Ethidium bromide-stained rRNA and GAPDH were used as loading controls. C, Visible phenotypes of 3-week-old wild-type, bt2-1, and BT2OE lines grown on MS medium containing H₂O₂ (2 mm). Total fresh weight (mg) of 30 seedlings from two different plates and ratio of weights relative to the wild type (WT; in parentheses) are shown on the right.