Figure 5.

Analysis of the transcriptional properties of Pax8 splicing isoforms. (A) Northern blot analysis of total RNA prepared from PCPy cells stably transfected with Pax8 splicing isoforms. Tg expression was analyzed in two different pools of clones for each transfected DNA. The GAPDH hybridization was used to normalize the amount of RNA present in each lane. (B) Reverse transcription–PCR analysis of cDNA derived from total RNA from the same cells as in A. Pax8 expression was analyzed in two different pools of clones for each transfected DNA.