Figure 1.

(A) Cartoon of bundlin structure. Protein tertiary structure is shown in the ribbon and wire format, whereas amino acids targeted for mutation in this study are shown in the stick format. Amino acids are colored according to the block in which they were mutated. Cartoon was produced in SwissPbdViewer, version 3.7 from the NMR-solved α₁ bundlin structure, reference number 1ZWT. (B) Predicted amino acid sequences for α₁, α₃, β₁- β₃, and β₆ bundlins at the domains mutated in this study. Coloured boxes around each domain correspond to the color of the amino acids in panel A. Amino acid number is given for the mature bundlin protein in each case. Dots represent invariant amino acids and dashed lines represent absent amino acids relative to the α₁ allele.