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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Mol Microbiol. 2009 Apr 14;72(4):859–868. doi: 10.1111/j.1365-2958.2009.06679.x

Figure 2.

Figure 2

Early LA of UMD901 strains complemented with WT and mutated α1 bfpA or WT β6 bfpA to HEp-2 cells in the presence of 280 µM LacNAc-BSA (light gray bars) or BSA (dark gray bars). Prior to each experiment, EPEC were incubated at 37°C in DMEM to induce BFP expression, and subsequently with 280 µM LacNAc-BSA or BSA. Bacteria were then incubated with sub-confluent HEp-2 cells for 45 minutes, followed by vigorous washing in PBS and enumeration by oil immersion light microscopy, as described previously (Vanmaele et al., 1999). X-axis labels represent bundlin type cloned into UMD901. Bars represent the mean of three experiments, and error bars indicate the standard deviations from the mean.