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Flow simulation of the gated flow chamber at equilibrium. (a) Flow in the chamber with gates and velocity distribution in transversal (A-A’ (b)); longitudinal (B-B’ (c)) directions. The flow rate at the inlet is 10 μL∕h. Due to the gated design, the velocity profile inside the culture chamber is uniform over 90% of the culture area. The velocity was calculated at 2 μm above the hole-side and cover-side surface (z=2 μm).
