Fig. 1.

Process for generating two-dimensional B lymphocyte arrays. (a) Fabrication procedure for creating the protein immobilization template. HMDS monolayer was coupled onto hydroxyl-group terminated glass substrates through silylation reactions and patterned by photolithography. The non-HMDS covered areas were passivated with PEG–silane to prevent the non-specific protein binding. (b) Schematic representation of molecular structures of PEG and HMDS after reacting with the surface hydroxyl groups. (c) Illustration of protein immobilization scheme for generating large area two-dimensional B lymphocyte arrays.