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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cell Signal. 2009 May 22;21(10):1488–1494. doi: 10.1016/j.cellsig.2009.05.007

Fig. 3. TAK1 is not required for LPA and PMA/Iono-induced IKK-NF-κB activation.

Fig. 3

(A and C) TAK1-/- MEF cells reconstituted with empty vector and TAK1 were either untreated or treated with LPA (A) or PMA/Iono (C) for 0, 30, and 60 min, then harvested. WCL and nuclear extracts were subjected to SDS-PAGE and immunoblotting analysis as indicated. (B and D) TAK1-/- MEF cells reconstituted with empty vector and TAK1 expression plasmid were either untreated or treated with LPA (B) or PMA/Iono (D) for 0, 5, 15, 30, and 60 min, then harvested. WCL were subjected to SDS-PAGE and immunoblotting analysis as indicated.