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Interaction of pyrene S265C G-actin with Bnr1. Panel A, pyreneS265C Mg-G-actin, 1 μm, was mixed with Bnr1 (line 1: no formin and line 2: 1 μm) in Mg- G-buffer at room temperature for 15 min, and the fluorescence emission spectra were recorded from 370–600 nm following excitation at 365 nm. Panel B, normalized net change in fluorescence at 485 nm from the titration was plotted against the concentration of Bnr1 in each reaction. The data are averaged from three individual experiments.
