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EM analysis of Mg-F-actin mixed with either Bnr1 or Bni1. 1 μm Mg-actin was polymerized in the presence of 0.25 μm of either Bnr1 (panels A and B) or Bni1 (panel C) at room temperature for 30 min. The product from each reaction was examined by EM as described under “Experimental Procedures.” Panel B, enlarged Bnr1/F actin bundle compared with the bundle in panel A. Bar = 0.2 μm.
