FIGURE 2.

Phosphorylation site-dependent dephosphorylation of Tau. A, two-dimensional phosphopeptide maps of phosphorylated WT, P301L, and R406W Tau before (0 min) and after a 10-min incubation with the rat brain extract. The radioactivity applied to cellulose plates was adjusted to 1000 cpm. The quantification of the respective spots in P-WT Tau and P-P301L is shown in (B). The relative signal intensity of the Ser-202, Ser-235, and Ser-404 phosphorylation sites is expressed as a fraction of the total signals of these phosphorylation spots in WT and P301L before (0 min) and after (10 min) dephosphorylation. These data are representative of those obtained from three different experiments.