FIGURE 5.

Effect of Pin1 on dephosphorylation of P-Tau in the absence or presence microtubules. A, autoradiograms of P-WT Tau on microtubules (MT) incubated with Pin1-KO mouse brain extract supplemented with 2 μm purified Pin1 (+, lanes 5 and 6) or not (−, lanes 3 and 4) for 0 or 10 min. Dephosphorylation of free P-WT Tau is shown in lanes 1 and 2. B, dephosphorylation of P-WT Tau, P-P301L, or P-R406W in the absence (−) or presence (+) of microtubules (MT) by Pin1-KO mouse brain extract supplemented with Pin1 (+) or not (−). Dephosphorylation of free Tau is shown in the left bar of each set of data. Dephosphorylation is expressed as the percentage of the extent of WT Tau dephosphorylation by Pin1-KO mouse brain extract in the absence of microtubules (MT). p values were calculated using Student's t test, and the bars indicate the means ± S.E. of three experiments; *, p < 0.01.