FIGURE 6.

Decreased hepatic Cyp7a1 mRNA and protein expression in chow-fed Apobec-1^−/− mice and a further decrease in LD-fed mice. A, relative expression of hepatic Cyp7a1 mRNA in chow-fed mice. Bar graphs show the mean ± S.E. of data from four WT and five Apobec-1^−/− mice. B, relative expression of hepatic Cyp7a1 mRNA in LD-fed mice. Bar graphs show the mean ± S.E. of data from three WT and five Apobec-1^−/− mice. C, Western blot analysis of CYP7A1 protein expression in liver microsomes prepared as described from chow or LD-fed animals. 25 μg of protein was subjected to SDS-PAGE and Western blotted with anti-CYP7A1 IgG and anti-BIP IgG. 3–4 mice from each genotype were studied.