TABLE 3.
Liver microarray data for lithogenic diet for 2 weeks
| Gene name | Gene description | Accession number | Ratioa | qRT-PCR | p |
|---|---|---|---|---|---|
| Bile acid synthesis, transport, and metablolism | |||||
| Cyp7a1 | Cholesterol 7α-hydroxylase | NM_007824 | 0.16 | 0.04b | 0.001 |
| Cyp8b1 | Sterol 12α-hydroxylase | NM_010012 | 0.36 | 0.07 | 0.01 |
| Cyp27a1 | Sterol 27-hydroxylase | NM_024264 | 0.82 | 0.9 | |
| Bsep/Abcb11 | Bile salt export pump | NM_021022 | 2.89 | 1.74 | |
| Ntcp | Na+-taurocholate co-transporting protein | NM_011387 | 1.7 | 0.63 | |
| Oatp4 | Organic anion transporter, 1b2 | NM_178235 | 2.01 | 0.48 | |
| Oatp2 | Organic anion transporter, 1a4 | NM_030687 | 3.49 | 2.55 | |
| Mdr2/Abcb4 | ATP-binding cassette, B4 | NM_008830 | 1.3 | 0.87 | |
| Mrp2/Abcc2 | ATP-binding cassette, C2 | NM_013806 | 1.13 | ||
| Cyp2b10 | Cytochrome P450, 2b10 | NM_009998 | 5 | 19.56 | |
| Cyp3a11 | Cytochrome P450, 3a11 | NM_007818 | 2.5 | 4.29 | |
| Cholesterol synthesis and transport | |||||
| Hmgcr | Hydroxymethylglutaryl-CoA reductase | NM_008255 | 2.09 | 1.84b | 0.04 |
| Abca1 | ATP-binding cassette, A1 | NM_013454 | 2.1 | 1.1 | |
| Abcg5 | ATP-binding cassette, G5 | NM_031884 | 1.6 | 1.03b | |
| Abcg8 | ATP-binding cassette, G8 | NM_026180 | 1.01 | 1.03 | |
| Srb1 | Scavenger receptor class, B1 | NM_016741 | 1.6 | 0.89 | |
| Scp2 | Sterol carrier protein 2, liver | NM_011327 | 2.1 | 1.71 | |
| Lfabp | Liver fatty acid binding protein | NM_017399 | 2.3 | 1.32 | |
| Cav1 | Caveolin 1 | NM_007616 | 1.8 | 0.69 | |
| Npc1 | Niemann-Pick type C1 | NM_008720 | 1.2 | ||
| Transcription factors or nuclear receptors | |||||
| Lxrα | Liver X receptor α | NM_013839 | 0.88 | 0.53b | |
| Fxr | Farnesoid X receptor | NM_009108 | 2.05 | 1.3 | |
| Shp | Small heterodimer partner | NM_011850 | 0.93 | 1.4 | |
| Pparα | Peroxisome proliferator-activated receptor α | NM_011144 | 2.1 | 1.1 | |
| Srebp1 | Sterol regulatory element-binding protein 1 | NM_011480 | 3.64 | 0.91b | |
| Srebp2 | Sterol regulatory element-binding protein 2 | NM_033218 | 0.61 | 0.83 | |
| Pxr | Pregnane X receptor | NM_010936 | 1.7 | 2.25 | |
| Lrh-1 | Liver receptor homolog | NM_030676 | 1.6 | ||
| Fatty acid synthesis and oxidation | |||||
| Fasn | Fatty acid synthase | NM_007988 | 8.1 | 15.1 | |
| Ucp2 | Uncoupling protein 2 | NM_011671 | 0.46 | 0.13 | |
| Scd1 | Stearoyl-coenzyme A desaturase 1 | NM_009127 | 1.6 | 2.07 | |
| Scd2 | Stearoyl-coenzyme A desaturase 2 | NM_009128 | 2.0 | 1.56 | |
| Acc | Acetyl-coenzyme A carboxylase α | NM_133360 | 2.0 | ||
| Lipoprotein metabolism | |||||
| ApoB | Apolipoprotein B | XM_137955 | 1.45 | 0.91b | |
| ApoA4 | Apolipoprotein A-IV | NM_007468 | 2.45 | 2.4 | |
| ApoC3 | Apolipoprotein C-III | NM_023114 | 2.45 | 1.16 | |
| ApoC2 | Apolipoprotein C-II | NM_009695 | 2.43 | 0.84 | |
| Ldlr | Low density lipoprotein receptor | NM_010700 | 0.8 | 1.83 | |
| ApoA1 | Apolipoprotein A | NM_009692 | 1.0 | ||
| ApoE | Apolipoprotein E | NM_009696 | 1.2 | ||
| Others | |||||
| Cyp2C38 | Cytochrome P450, 2C38 | NM_010002 | 4.9 | 1 | |
| Cyp4a14 | Cytochrome P450, 4A14 | NM_007822 | 122.8 | 372.2 | |
a Microarray data are expressed as a ratio of Apobec1−/− versus WT. Relative mRNA expression of some genes was confirmed by real time qRT-PCR by using pooled cDNA (5 mice/group). qRT-PCR results are expressed as -fold change normalized to WT control.
b Relative mRNA expression of indicated genes was confirmed using individual samples (n = 4).