FIGURE 6.

Competition between LTBP-1 and fibrillin-1 for fibulins. Fibulin-2, fibulin-4, and fibulin-5 were each used to coat a BiaCore chip. A constant amount (80 nm) of recombinant fibrillin-1 (rF23) was preincubated with increasing amounts (50–800 nm) of LTBP-1 competitor (rL1K). Binding of rF23 without competitor is set as 100%, and results with competitor LTBP-1 are shown as a percentage of this maximal binding. Titrations of LTBP-1 show that LTBP-1 binding to fibrillin-1 competes with fibrillin-1 binding to fibulin-2, fibulin-4, and fibulin-5.