TABLE 1.
KD values (in nanomolar) of interactions determined by surface plasmon resonance
Analytes, listed in the column on the left, were titrated and flowed over chips coupled with the ligands listed in the top row. One experiment consisted of a set of titrations. Interactions that were not tested are listed as not determined. “No binding” indicates that all titrations yielded flat responses.
| rL1K | rL4K | Fibulin-2 | Fibulin-4 | Fibulin-5 | |
|---|---|---|---|---|---|
| rF23 | 21 ± 2 (3)a | 24 ± 5 (4) | 160 | 54 ± 6 (5) | 23 |
| rF23 BNPS cleaved | 213 ± 10 (3) | 72 | NDb | ND | ND |
| rF38N | 65 | ND | ND | ND | ND |
| rF68 | 26 ± 16 (5) | 44 ± 14 (2) | 1260 | 475 | 258 |
| rF79 | 23 ± 8 (2) | 70 | No binding | 67 | 62 |
| rF80 | 104 ± 40 (7) | No binding | No binding | 150 ± 50 (2) | 90 |
| rF23mut1 | 25 ± 5 (3) | 348 ± 221 (2) | 1530 | 430 | 298 |
| rF31 | No binding | ND | ND | No binding | No binding |
| rL1K | ND | ND | No binding | No binding | No binding |
| rL4K | ND | ND | ND | ND | No binding |
a Numbers of experiments that were performed are listed in parentheses.
b ND, not determined.