FIGURE 4.

Itraconazole treatment results in altered lectin binding to macrophage proteins. RAW 264.7 Mϕs were treated with itraconazole (1 μm) or the respective carrier (DMSO) for 16 h followed by ConA in the presence or absence of α-methylmannoside (A) or PHA-E (B) lectin blot analysis. Blots were stripped and re-probed for β-Actin as a loading control. Primary C57BL/6J peritoneal Mϕs were treated with itraconazole (5 μm) or the respective carrier (DMSO) for 16 h followed by ConA lectin blot analysis. The blot was stripped and re-probed for β-Actin as a loading control (C). RAW 264.7 Mϕs were treated with itraconazole (1 μm) or the respective carrier (DMSO) for 16 h followed by staining with fluorescein isothiocyanate-conjugated ConA (100 μg/ml) in the presence or absence of α-methylmannoside (α-MM) (1 mg/ml) and analyzed by flow cytometry (D).