TABLE 1.
Sterol analysis of RAW 264.7 macrophages
Cells were treated with itraconazole (1 μm), lovastatin (10 μm), NB-598 (30 μm), 5% lipoprotein-deficient serum or itraconazole (1 μm) + lovastatin (10 μm) or NB-598 (30 μm) or the respective carrier (DMSO) for 16 h. Sterols were saponified by incubation of cell pellets in MeOH, 60% KOH (2:1), extracted and analyzed by gas chromatography. Ergosterol was added as an internal standard, and samples were corrected by both ergosterol for extraction and protein levels for input.
| Treatment | Cholesterol | Lanosterol |
|---|---|---|
| Control | 11.42 ± 1.12 | |
| Carrier | 13.17 ± 1.42 | |
| Itraconazole (1 μm) | 11.69 ± 2.45 | 0.63 ± 0.16 |
| Lovastatin (10 μm) | 12.31 ± 1.14 | |
| NB-598 (30 μm) | 8.84 ± 0.83a | |
| Itraconazole + lovastatin | 11.17 ± 1.20 | |
| Itraconazole + NB-598 | 7.83 ± 0.22a | |
| 5% LPDS | 7.29 ± 0.45a |
a p < 0.05 versus control and carrier by t test.