FIGURE 1.

Confirmation of expression of SEPS1 and ZAAT by HepG2 transfectants. A, HepG2 cells (1 × 10⁶) were transfected with empty vector (lane 1) or pSEPS1 (lane 2). Protein extracts (40 μg) were analyzed by Western immunoblot. B, SEPS1 expression was quantified by quantitative reverse transcription-PCR. C, HepG2 cells were cotransfected with pRLSV40 and an empty vector (CMV) or pZAAT. ZAAT expression in cell lysates was quantified by an enzyme-linked immunosorbent assay. Absorbance at 405 nm per light unit (L.U.) indicates relative expression of ZAAT. The dashed line indicates background absorbance readings. Data shown are representative of three experiments.