FIGURE 5.

Glutathione peroxidase activity and SEPS1 expression in control and selenium supplemented cells. HepG2 cells were grown in complete medium containing 0 nm, 40 nm, or 150 μm seleno-dl-methionine, as indicated, for 72 h. A, a glutathione peroxidase cellular activity assay kit (Sigma) was used to measure total GPx activity. The result shown is representative of three repeated experiments. B, RNA was isolated and used in quantitative reverse transcription-PCRs to detect SEPS1 and glyceraldehyde-3-phosphate dehydrogenase expression. Relative SEPS1 expression is shown. C, protein extracts were prepared, and samples (10 μg) were immunoblotted for SEPS1 or stained with Ponceau S for total protein loading.