FIGURE 3.

NSC-741909-induced activation of MAP kinases. a, cells were treated with NSC-741909 (1 μm for H460; 10 μm for T29 and T29Kt1) for 0, 8, and 12 h. The whole-cell lysates were subjected to Western blot analysis to detect basal and phosphorylated JNK (p-JNK), ERK (p-ERK1/2), and P38 (p-P38). b, H460 cells were treated with 1 μm NSC-741909 for the indicated times. Cell lysates were then subjected to Western blot analysis. β-Actin was used as the loading control. c, H460 cells were treated for 6 h with the indicated concentrations of NSC-741909. Levels of phospho-JNK and JNK were detected by Western blot analysis. d and e, H460 cells (d) and H157 cells (e) were treated with different concentrations of NSC-741909 in the presence or absence of 10 μm SP600125 (JNK-specific inhibitor), 20 μm U0126 (MEK-specific inhibitor), or 20 μm SB203580 (P38 inhibitor). Cell viability was determined at 24 h after treatment. Cells treated with DMSO were used as a control, with viability set at 100%. Each data point represents the means ± S.D. of three independent experiments.