FIGURE 4.

Apoptosis induction by NSC-741909. Cells were treated 1 μm NSC-741909 in the presence or absence of 10 μm SP600125, 20 μm U0126, or 20 μm SB203580. Cells treated with DMSO or the MAP kinase inhibitors alone were used as controls (Ctrl). Apoptotic cells were determined by flow cytometric analysis at 24 h after treatment. a, the percentages of apoptotic cells. The values represent the means + S.D. of three analyses. **, p < 0.01, when compared with cells treated with NSC-741909 alone. b, an example of fluorescence-activated cell sorter histograms. c, H460 cells were treated with 1 μm NSC-741909 in the presence or absence of 10 μm SP600125. Whole-cell lysates were harvested for Western blot analysis of JNK activation and apoptosis at the indicated time points. p-JNK, phospho-JNK; p-cJUN, phospho-c-Jun; PARP, poly(ADP-ribose) polymerase.