FIGURE 5.

Effect of dominant-negative JNK-1 on NSC-741909-induced cell killing. H157 cells were transfected with a plasmid expressing dnJNK1 or a control vector. After selection with G418, two H157 clones expressing dnJNK1 were identified by Western blot analysis with anti-HA (an epitope tag from influenza hemagglutinin) antibody. a, parental, vector-transfected, or dnJNK1-transfected H157 cells were then analyzed for their susceptibility to NSC-741909. b, dose response to NSC-741909 as determined by the sulforhodamine B assay. Values represent the means ± S.D. from three independent experiments. c, apoptosis induction as determined by the flow cytometric assay. Cells were treated with 1 μm NSC-741909, and apoptosis was determined at 24 h after treatment. The numbers indicate the proportions of apoptotic cells.