TABLE 1.

List of top five proteins with the most decreased levels in the cytoplasmic fraction of H460 cells after 6 and 24 h of treatment with 15 μm PEITC

Two isoforms of actin are also listed for comparison. The percentage of level change relative to the untreated control was ranked as indicated in Fig. 2, A and B. The p value was included to indicate the statistical significance of the protein quantitation. The unused value is confidence measurement of protein identification where a value of 1 indicates 90% confidence, and a value of >2 indicates 100% confidence. Sequence coverage was calculated by dividing the number of amino acids observed by the protein amino acid length.

Level change ranking	Change in % of control	p value for quantitation	Protein name	NCBI accession number	Unused value	Sequence coverage
						%
6 h of treatment
1	−65.763	0.000494	Tubulin β chain	P07437	13.08	79.7
2	−56.687	0.013724	Tubulin β-2C chain	P68371	1.73	78.8
3	−49.941	9.88E-11	Tubulin α-1B chain	P68363	18.14	66.5
4	−46.716	0.084579	Neuroblastoma breakpoint family member 3	Q9H094	1.11	21.8
5	−39.480	0.047385	Tubulin α-1C chain	Q9BQE3	1.44	65.0
168	3.8304	0.188479	Actin, cytoplasmic 1	P60709	43.98	87.7
192	6.2065	0.136969	Actin, cytoplasmic 2	P63261	2.22	83.3
24 h of treatment
1	−67.135	0.000698	Tubulin β chain	P07437	13.08	79.7
2	−54.784	4.96E-11	Tubulin α-1B chain	P68363	18.14	66.5
3	−53.853	0.041881	Tubulin β-2C chain	P68371	1.73	78.8
4	−44.660	0.025489	Tubulin α-1C chain	Q9BQE3	1.44	65.0
5	−28.895	0.462542	Importin-4	Q8TEX9	2.09	23.9
69	−4.1809	0.010398	Actin, cytoplasmic 1	P60709	43.98	87.7
170	5.0402	0.148074	Actin, cytoplasmic 2	P63261	2.22	83.3