FIGURE 8.

p53 expression and phosphorylation are increased in normoxia and potentiated in hypoxia in 6-AN-treated cells. A, HEK293T cells were pretreated with 6-AN (100 μm) for 2 h followed by exposure to 16 h in hypoxia (0.2% oxygen). Cell lysates were prepared, and p53 Western analysis was performed as described under “Experimental Procedures.” Con, control. B, oxidation of Trx increased in 6-AN-treated cells in normoxia and is potentiated in 6-AN-treated cells exposed to hypoxia. MCF-7 cells were treated with 6-AN (50 μm), and following exposure oxidized and reduced Trx was analyzed as described under “Experimental Procedures.” Upper panel, densitometry of oxidized band; lower panel, Western analysis of Trx oxidation state.